[Care concepts in mass casualty incidents and disasters. Concept for primary care clinic]. / Versorgungskonzepte im Großschadens- und Katastrophenfall. Das Konzept der Erstversorgungsklinik.

BACKGROUND: Patient care in mass casualty incidents and disaster strongly demand a joint approach of all preclinical and clinical forces. OBJECTIVE: Special emphasis must be placed on immediate triage, establishment and preservation of transportability of high-risk patients and their clinical treatment as soon as possible. During limited mass casualties, the preclinical rescue station additionally serves as a buffer for patients, whereby in case of disaster, the focus on transportation of high-risk patients is imperative. DISCUSSION AND CONCLUSION: Primary care hospitals are a decisive part in the chain of medical supply and are confronted with great challenges, which demand detailed emergency plans and also repeated exercises. In planning and exercises, special attention should be given to the cooperation with the fire department and other medical services.

[The hospital emergency plan]. / Der Notfallplan des Krankenhauses.

STRUCTURE: The hospital emergency plan consists of a basic plan and an appendix. The basic plan deals with the general aspects of emergency operation and the special aspects of external and internal emergencies. The appendix contains special instructions, e.g., emergency action orders, staff alert lists, material lists, and situation plans. CONTENT AND IMPLEMENTATION: External emergencies (e.g., mass casualty incidents) and internal emergencies (e.g., fire and other environmental threats) should be regarded. Once a defined experienced physician decides to activate the emergency plan, the hospital changes from routine to emergency service. Due to its security significance, the emergency plan should be regarded as confidential. MANDATORY REQUIREMENTS: A two-tier chain of command is implemented: the hospital staff deals with administrational and organizational aspects, whereas the subsequent medical staff is in charge of immediate patient care. Repeated updating and exercises are necessary. Furthermore, a basic logistic autonomy of the hospital is essential.

[Circulation therapy for severe burn injuries]. / Kreislauftherapie beim Schwerbrandverletzten.

Patients with burn injuries to more than 10% of the body surface area (BSA) are in potential danger of traumatic hypovolemic shock and from 20% BSA a generalized burn edema can occur. In the preclinical setting an increased infusion therapy is generally unnecessary. Clinical circulation therapy is goal-directed taking hemoglobin concentration, hematocrit, MAP, diuresis, CVP and central venous sO(2 )into consideration. For initial volume replacement, balanced crystalloids with the addition of acetate and possibly malate are infused. Colloids should be given with great caution. Additional gelatine solution is only to be used in patients with impending hypotension and catecholamines should also be avoided if possible. If necessary, dobutamine is used to increase cardiac inotropy and cardiac output. Norepinephrine is only indicated in patients with significantly reduced SVR. Extended hemodynamic monitoring is necessary in all patients with prolonged catecholamine therapy.

[Primary care hospital for a mass disaster MANV IV. Experience from a mock disaster exercise]. / Die Erstversorgungsklinik bei einem Grossschadensereignis MANV IV. Erfahrungen aus einer Vollübung.

BACKGROUND: In Hannover and in nationwide contingency plans there are clear instructions for the medical care of mass casualties which are designed to cope with 50 to a maximum of 200 patients. Disaster simulations and practical exercises in Hannover regarding EXPO 2000 and the FIFA World Cup 2006 showed a very good and effective prehospital treatment and management up to a number of about 200 patients. Due to infrastructural settings a scenario with up to 1,000 (MANV IV) patients in the region of Hannover was beyond the capacity of existing concepts for the management of mass casualties, which comprised initial medical care at the on-site treatment area and subsequent transport to local or regional hospitals for definitive management. A new practicable and well trained model was necessary to improve the hospital admission and primary treatment capacity (Erstversorgungsklinik--EVK). In the case of MANV IV it was proposed that the tasks of on-site treatment area should be concentrated on triage and the stabilization of severely injured victims with immediate transport to special primary care hospitals. The main task of these hospitals was further stabilization of patients for inhospital care or further transport to other special facilities. METHODS: The main aim of the study was, after the initial trauma scenario, to provide the logistical and personal background for the fastest possible advanced life support and the further treatment of more than 60 severely injured patients at a city hospital with trauma centre level I experience. The timescale from the first alarm until the hospital was ready for action was approximately 60 min. To gain knowledge about the regional implementation of the whole logistic scenario in the case of MANV IV and to practice detailed questioning, a major casualty training was needed. This resulted in a large targeted disaster medical training with a realistic situation simulation on the 25.03.2006 including the Diakoniekrankenhaus Friederikenstift under the aspect of a special primary care hospital (EVK) working at full capacity. RESULTS: The AWD arena in Hannover was the site of a simulated major casualty event resulting in 620 patients with various penetrating or blunt trauma injuries. Within 60 min of the first alarm call the admission and casualty treatment capacity at the Diakoniekrankenhaus Friederikenstift was increased up to approximately 60 patients including 30 ventilated patients. After initial inspection of 78 patients according to the ATLS criteria advanced life support was performed (airway management, volume resuscitation, basic diagnostic and surgical techniques) by flexible treatment teams (including physicians of all other faculties) in 3 treatment corridors within 135 min. Of the patients 69 were admitted to the wards and intensive care units, 5 were discharged after ambulant treatment and 3 patients were transferred to an eye and ENT hospital. Of the patients 10 had already been intubated on arrival, another 6 patients were intubated in the treatment corridors. Simulations of 4 urgent laparatomies, 2 trepanations, 1 artery seam, osteosynthesis of 3 perforating fractures was performed in the operating theatre. A total of 6 extremity fractures were immobilized by a fixateur externe, 7 chest tubes were placed and 43 surgical wound dressings were performed in the treatment corridors. There was no significant shortage of logistical or personal resources. CONCLUSION: In a major disaster with more than 200 seriously injured patients the EVK model is a practicable and regional well tried solution that could increase the capacity of hospital admissions and advanced trauma life support, regardless of the type of casualty, season or weather conditions. It is possible to reduce the interval to advanced trauma life support, temporary fracture stabilization (damage control) and definitive surgical care by means of rapid and targeted utilization of resources and manpower. Physicians involved in the initial treatment play a key role and have to be highly trained (ATLS). The EVK model is variable and can easily be established and adapted to regional conditions at basic regional hospitals as well as at level I trauma centers.

Regulation of brain gene expression in honey bees by brood pheromone.

Pheromones are very important in animal communication. To learn more about the molecular basis of pheromone action, we studied the effects of a potent honey bee pheromone on brain gene expression. Brood pheromone (BP) caused changes in the expression of hundreds of genes in the bee brain in a manner consistent with its known effects on behavioral maturation. Brood pheromone exposure in young bees causes a delay in the transition from working in the hive to foraging, and we found that BP treatment tended to upregulate genes in the brain that are upregulated in bees specialized on brood care but downregulate genes that are upregulated in foragers. However, the effects of BP were age dependent; this pattern was reversed when older bees were tested, consistent with the stimulation of foraging by BP in older bees already competent to forage. These results support the idea that one way that pheromones influence behavior is by orchestrating large-scale changes in brain gene expression. We also found evidence for a relationship between cis and BP regulation of brain gene expression, with several cis-regulatory motifs statistically overrepresented in the promoter regions of genes regulated by BP. Transcription factors that target a few of these motifs have already been implicated in the regulation of bee behavior. Together these results demonstrate strong connections between pheromone effects, behavior, and regulation of brain gene expression.

Advancing the understanding of the embryo transcriptome co-regulation using meta-, functional, and gene network analysis tools.

Embryo development is a complex process orchestrated by hundreds of genes and influenced by multiple environmental factors. We demonstrate the application of simple and effective meta-study and gene network analyses strategies to characterize the co-regulation of the embryo transcriptome in a systems biology framework. A meta-analysis of nine microarray experiments aimed at characterizing the effect of agents potentially harmful to mouse embryos improved the ability to accurately characterize gene co-expression patterns compared with traditional within-study approaches. Simple overlap of significant gene lists may result in under-identification of genes differentially expressed. Sample-level meta-analysis techniques are recommended when common treatment levels or samples are present in more than one study. Otherwise, study-level meta-analysis of standardized estimates provided information on the significance and direction of the differential expression. Cell communication pathways were highly represented among the genes differentially expressed across studies. Mixture and dependence Bayesian network approaches were able to reconstruct embryo-specific interactions among genes in the adherens junction, axon guidance, and actin cytoskeleton pathways. Gene networks inferred by both approaches were mostly consistent with minor differences due to the complementary nature of the methodologies. The top-down approach used to characterize gene networks can offer insights into the mechanisms by which the conditions studied influence gene expression. Our work illustrates that further examination of gene expression information from microarray studies including meta- and gene network analyses can help characterize transcript co-regulation and identify biomarkers for the reproductive and embryonic processes under a wide range of conditions.

[Hospital emergency department preparedness for NBC mass casualties]. / Der Notfallplan des Krankenhauses bei ABC-Gefahrenlagen.

Hospital emergency department preparedness for mass-casualty incidents involving nuclear, biological or chemical (NBC) threats relies on close cooperation between hospital and pre-hospital emergency staff. It is essential that the hospital is immediately secured from unauthorized intrusion in order to avoid contamination of the hospital area and staff. The strategy of the pre-hospital emergency staff to avoid the unnecessary spread of contaminated material involves thorough decontamination of exposed persons near the site of the incident and coordinated transport to the primary care hospitals after decontamination. However, uncoordinated access of contaminated victims requires emergency decontamination by hospital staff. Thus, hospital staff must be prepared to provide in-hospital decontamination. Coordinated admission of contaminated patients into the NBC primary care hospital relies on a thorough decontamination by pre-hospital emergency staff at a decontamination site installed outside the hospital. Screening of patients is performed by hospital staff with special expertise in emergency medicine. Following admission, each patient is assigned to a team of specialists. Pre-hospital patient documentation is switched to inhospital documentation after admission using machine-readable electronic admission numbers.

Endocrine stress response and inflammatory activation during CABG surgery. A randomized trial comparing remifentanil infusion to intermittent fentanyl.

BACKGROUND AND OBJECTIVE: Our aim was to compare a continuous infusion of remifentanil with intermittent boluses of fentanyl as regards the perioperative hormonal stress response and inflammatory activation in coronary artery bypass graft patients under sevoflurane-based anaesthesia. METHODS: In all, 42 patients undergoing coronary artery bypass grafting with cardiopulmonary bypass were prospectively randomized to a fentanyl group (n = 21, total fentanyl dose 2.6 +/- 0.3 mg), or a remifentanil group (n = 21, infusion rate 0.25 microg kg(-1) min(-1)). Haemodynamics, plasma levels of epinephrine, norepinephrine, antidiuretic hormone, adrenocorticotropic hormone, cortisol, complement activation (C3a, C5b-9), interleukin (IL)-6, IL-8 and tumour necrosis factor-alpha were measured at T1: baseline, T2: intubation, T3: sternotomy, T4: 30 min on cardiopulmonary bypass, T5: end of surgery and T6: 8 h postoperatively. Troponin T and creatine kinase-MB were measured postoperatively. RESULTS: Patients in the remifentanil group were extubated significantly earlier than fentanyl patients (240 +/- 182 min vs. 418 +/- 212 min, P = 0.006). Stress hormones 30 min after start of cardiopulmonary bypass showed higher values in the fentanyl group compared to the remifentanil group (antidiuretic hormone (ADH): 39.94 +/- 30.98 vs. 11.7 +/- 22.8 pg mL(-1), P = 0.002; adrenocorticotropic hormone: 111.5 +/- 116.8 vs. 21.81 +/- 24.71 pg mL(-1), P = 0.01; cortisol 185 +/- 86 vs. 131 +/- 82 ng mL(-1), P = 0.04). The interleukins were significantly higher at some perioperative time points in the fentanyl group compared to the remifentanil group (tumour necrosis factor: T5: 3.57 vs. 2.37; IL-6: T5: 4.62 vs. 3.73; and IL-8: T5: 4.43 vs. 2.65 and T6: 2.61 vs. 1.13). However, cardiopulmonary bypass times and aortic cross-clamp times were longer in the fentanyl group, which may to some extent account for the differences. CONCLUSIONS: The perioperative endocrine stress response was attenuated in patients supplemented with continuous remifentanil infusion as compared to intermittent fentanyl.

[Hemodilution and infusion therapy for hypovolemic shock. Clinical physiological and pharmacological aspects]. / Hämodilution und Infusionstherapie bei hypovolämischem Schock. Klinisch-physiologische und pharmakologische Aspekte.

Hypovolemic shock is not a form of disease and can be subdivided into four special types with varying therapeutic demands. The decisive approach in the therapy of hypovolemic shock is to initially attain normovolemia by rapid administration of volume replacement agents in the sense of controlled hemodilution. This allows an adequate increase in the cardiac output resulting in delivery of sufficient oxygen to tissues. In the following article the limits of intervention will be described and the advantages and disadvantages of these measures for patients suffering from hypovolemic shock will be critically considered.

[The hospital emergency plan - basics and general structure]. / Der Notfallplan des Krankenhauses: Grundlagen und allgemeine Struktur.

A hospital emergency plan commonly consists of three main chapters dealing with the general aspects of emergency operation and the special aspects of external and internal emergencies. The extent of these chapters is restricted to general understanding and is completed by special instructions (emergency action plan, staff lists, material lists, situation plans) for each department. Due to its security significance, the emergency plan is handled confidential. External emergencies include mass casualty incidents, incidents with a large number of intoxicated patients, the care of patients with dangerous infectious diseases and NBC emergencies. Internal emergencies include fire and other environmental threats, the breakdown of the electrical power and water supply, the breakdown of the telephone system and other situations like bomb threat and taking of hostages. Any emergency call is sent to a special emergency phone, and a defined, experienced physician decides to activate the emergency plan. With the change from routine to emergency service, a chain of command is installed. The hospital staff deals with administrational and organizational aspects, whereas the medical staff is in charge of immediate patients' care. For each external emergency, the deployment of personnel and material and the basic organizational aspects are ordered. Comparable preparations are given for internal emergencies. To achieve realistic emergency planning, repeated evaluations and practical exercises are necessary. Furthermore, a basic independence of the hospital with respect to stocks, preparation and sterilization of instruments and catering is essential to ensure its function even under adverse conditions.

[Hospital emergency plan for the management of patients with highly contagious diseases]. / Der Notfallplan des Krankenhauses bei allgemeingefährlichen Infektionskrankheiten.

Patients with imported highly contagious diseases like viral hemorrhagic fever (VHF), pneumonic plague or anthrax require special preventive measures and strict isolation. In addition, new emerging infections like severe acute respiratory syndrome (SARS) and epidemic influenza also have to be taken into account. At present five high security infectious disease units for patient care are available in Germany. Due to the relatively nonspecific symptoms, a patient with one of these infections may also be admitted to any other hospital. Therefore each hospital should develop a concept for the management and control of these lifethreatening highly contagious diseases. The present article describes the current emergency plan developed for the management of patients with highly contagious diseases at Hannover Medical School.

[Requirements and expectations for optimal volume replacement]. / Forderungen und Erwartungen an einen optimalen Volumenersatz.

A volume replacement should compensate a reduction in the intravascular volume and counteract a hypovolemia so that hemodynamics and vital functions can be maintained. For this therapy, a physiologically-based solution comprising both osmotically and colloid osmotically active components should be administered. A consensus is proposed for this purpose which takes into consideration the following aspects: The optimum colloid, the questionable use of albumin, the physiological electrolyte pattern encompassing sodium, potassium, chloride and phosphate and their contributions to osmolality, an eventual addition of glucose, the physiological acid-base status with bicarbonate or alternately with metabolisable anions, and the importance of a clear declaration of all ingredients. The consensus distinguishes between compulsory requirements derived from evidence-based medicine and physiological data and the potential expectations of an optimal volume replacement, including well-grounded wishes and aspirations for the future.